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KMID : 0380020060210040306
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.306 ~ p.311
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Short-term Hypothermic Preservation of CHO Cells Using Serum-Free Media
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Byun Soon-Hyu
Park Hong-woo
Choe Tae-Boo
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Abstract
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Cell preservation is indispensable in animal cell culture process and should be established according to the cell characteristics. In this study, we experimented hypothermic preservation of CHO cells that is widely used in pharmaceutical industry to produce therapeutic proteins and established a stable method of preservation. The highest viability of CHO cells was obtained when the cells were preserved using rolling tube, which means the cells should be suspended to avoid the cell lumping during the preservation. Also, we obtained superior preservation result under the anaerobic condition. To evaluate the serum-free media as a preservation solution, we investigated cell growth after hypothermic preservation using serum-free media. High cell viability and normal cell growth was observed during 10 days using serum-free media. Moreover, we found that more effective preservation when α-tocopherol and retinoic acid is added to media as an additive. In the case of 1 liter large scale hypothermic preservation using established protocol, cell viability and growth rate was obtained as good as small scale one. This study is considered to be helpful for hypothermic preservation of CHO cells and large scale hypothermic preservation may be available through the further studies.
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KEYWORD
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Hypothermic preservation, CHO cells, serum-free media
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